Spore Based Kit for Detection of Antibiotic Residues in Milk at Dairy Farm

Technology Details:
The developed technology is working on principle of spore germination and its inhibition in presence of antibiotic residues in milk. In case when antibiotic residues are absent in milk, marker metabolites are released during germination which change the color of the indicator. However, in presence of antibiotic residues in milk, the spore germination process is inhibited at ≥ MRL level of contaminants and no change in color indicates the presence of drug residues in milk when incubated at 64 ?C for 2.30 hrs. Result interpretation: Colour changes from purple to yellow indicate absence of antibiotic residues while persistence of purple color indicates presence of antibiotic residues ≥ MRL level.

• The Cost effective (Rs 35 per test).
• Semi-quantitative detection of β-Lactam group, aminoglycosides, tetracycline, chloramphenicol, macrolides and sulfa drugs at Codex MRL.
• Validated with AOAC approved Charm 6602 Assay.
• Minimal false positive / negative results.
• No interference of inhibitors other than antibiotic residues.
• Stability of test kits up to 12 months under refrigeration storage.
• Test kit can be applied at dairy farm, milk collection center, dairy reception dock and R&D institutions etc.